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25nmoles起订
产品规格
可售数量： 25nmoles
产品参数
| Ex (nm) | - | Em (nm) | - |
| 分子量 | 898.71 | 溶剂 | Water |
| 存储条件 | 在零下15度以下保存, 避免光照 |
简要概述
产品基本信息
货号：17015
产品名称：生物素-11-dGTP
规格：25 nmoles
储存条件：-15℃避光防潮
保质期：24个月
产品物理化学光谱特性
分子量：898.71
溶剂：水
产品介绍
生物素修饰的脱氧鸟苷5'-三磷酸酯广泛用于各种非放射性DNA标记反应，包括切口平移，随机引物标记，cDNA标记和3'-末端标记。可以通过切口平移，随机引物，3'-末端末端标记或PCR的方法将生物素11-dGTP酶促地掺入DNA中。数字“ 11”是dGTP与生物素之间连接子骨架中的碳原子数。连接子越长，生物素与抗生物素蛋白的相互作用越有效。另一方面，接头越短，dGTP就越有效地掺入DNA。建议链接器'11'的长度对于大多数应用而言是很好的。 Biotin-11-dGTP用于在各种杂交应用中产生生物素化的DNA探针，包括Southern印迹，Northern印迹，斑点印迹，固定细胞和组织。它在化学上等同于PerkinElmer（PE）的NEL541001EA。
参考文献
Base-Excision-Repair-Induced Construction of a Single Quantum-Dot-Based Sensor for Sensitive Detection of DNA Glycosylase Activity.
Authors: Wang, Li-Juan and Ma, Fei and Tang, Bo and Zhang, Chun-Yang
Journal: Analytical chemistry (2016): 7523-9
PRINS for mapping single-copy genes.
Authors: Tharapel, Avirachan T and Wachtel, Stephen S
Journal: Methods in molecular biology (Clifton, N.J.) (2006): 59-67
Chromosomal localization of single copy genes SRY and SOX3 by primed in situ labeling (PRINS).
Authors: Kadandale, J S and Tunca, Y and Tharapel, A T
Journal: Microbial & comparative genomics (2000): 71-4
Quantification of telomerase activity by direct scintillation counting.
Authors: Kazmer, S and Pan, K M and Vassilev, L
Journal: Journal of biochemical and biophysical methods (1999): 113-7
Telomerase assay using biotinylated-primer extension and magnetic separation of the products.
Authors: Sun, D and Hurley, L H and Von Hoff, D D
Journal: BioTechniques (1998): 1046-51
Universal cloning and direct sequencing of rearranged antibody V genes using C region primers, biotin-captured cDNA and one-side PCR.
Authors: Heinrichs, A and Milstein, C and Gherardi, E
Journal: Journal of immunological methods (1995): 241-51
Purification of DNA fragments containing excision-repair patches from human cells using streptavidin-biotin.
Authors: Larone, G E and Hunting, D J
Journal: Mutation research (1991): 273-80
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18502920169
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029-68064558
AAT Bioquest 生物素-11-dGTP 货号17015
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25nmoles可售
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