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iFluor 488-dUTP * 1 mM的Tris缓冲液(pH 7.5)*
货号 | 17039 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 25 nmoles | |||
Ex (nm) | 491 | Em (nm) | 516 | |
分子量 | 1152.83 | 溶剂 | Water | |
产品详细介绍 |
产品物理化学光谱特性
分子量:1152.83
溶剂:水
激发波长(nm):491
发射波长(nm):516
产品介绍
染料修饰的脱氧尿苷5'-三磷酸酯是通过常规酶掺入方法(例如反转录,缺口翻译,随机引物标记或PCR)生产染料标记DNA的常用方法之一。 这种酶促荧光标记方法广泛用于FISH探针和基于微阵列的实验。 该iFluor 488-dUTP缀合物与SpectrumGreen 滤光片组合一起用作绿色荧光(SpectrumGreen 是Vysis的商标)。 与常用的绿色探针(例如Fluorescein-12-dUTP)相比,iFluor 488提供了更明亮的信号,具有很高的光稳定性,并且不受pH值的影响。
参考文献
Anti-proliferative effect of Fe(III) complexed with 1-(2-hydroxy-3-methoxybenzaldehyde)-4-aminosalicylhydrazone in HepG2 cells.
Authors: Fukushima, Takeshi and Taniguchi, Erina and Yamada, Hiroshi and Kato, Kiyomasa and Shimizu, Ayako and Nishiguchi, Yoshikazu and Onozato, Mayu and Ichiba, Hideaki and Azuma, Yutaro
Journal: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine (2015): 669-77
Localisation of abundant and organ-specific genes expressed in Rosa hybrida leaves and flower buds by direct in situ RT-PCR.
Authors: Jedrzejuk, Agata and Mibus, Heiko and Serek, Margrethe
Journal: TheScientificWorldJournal (2012): 609597
SNP genotyping using microsphere-linked PNA and flow cytometric detection.
Authors: Rockenbauer, Eszter and Petersen, Kenneth and Vogel, Ulla and Bolund, Lars and Kølvraa, Steen and Nielsen, Kirsten Vang and Nexø, Bjørn A
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2005): 80-6
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18502920169
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029-68064558
AAT Bioquest iFluor 488-dUTP * 1 mM的Tris缓冲液(pH 7.5)* 货号1703
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