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产品规格
可售数量: 5mg
产品参数
Ex (nm) | 491 | Em (nm) | 516 |
分子量 | N/A | 溶剂 | DMSO |
存储条件 | 在零下15度以下保存, 避免光照 |
产品概述
产品基本信息
产品名称:ReadiCleave iFluor 488 SSL-NHS酯
储存条件:-15℃避光防潮
保质期:12个月
产品物理化学光谱特性
分子量:1064.14
外观:固体
溶剂:DMSO
激发波长(nm):491
发射波长(nm):516
产品介绍
基于荧光的方法在灵敏度和便捷性方面对生物检测具有诸多优势,许多生物分子可以很容易地用荧光标记物进行荧光成像和流式细胞分析。然而,现有的荧光标记物大多用标记生物靶标,无法将加入的荧光标记物切割下来用于进一步的下游分析,如质谱分析或其他检测模式。AAT Bioquest公司的Readi Cleave 探针使荧光标记与生物靶标结合,在需要时可以将添加的荧光标记从生物靶标上移除。ReadiCleave™iFluor™488 SSL - NHS酯含有一个S - S连接子,可以与TCEP或DTT切割,以去除目标分子中的iFluor 488荧光团。与传统的FITC染料相比,iFluor 488具有几乎相同的Alexa Fluor 488光谱,具有高荧光量子产率和改善的稳定性。加入DTT或TCEP溶液,室温至65℃孵育1 - 10 min即可进行切割。
溶解方案(溶剂以说明书为准)
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 93.973 µL | 469.863 µL | 939.726 µL | 4.699 mL | 9.397 mL |
5 mM | 18.795 µL | 93.973 µL | 187.945 µL | 939.726 µL | 1.879 mL |
10 mM | 9.397 µL | 46.986 µL | 93.973 µL | 469.863 µL | 939.726 µL |
参考文献
Release Mechanisms of Amorphous Solid Dispersions: Role of Drug-Polymer Phase Separation and Morphology.
Authors: Yang, Ruochen and Zhang, Geoff G Z and Zemlyanov, Dmitry Y and Purohit, Hitesh S and Taylor, Lynne S
Journal: Journal of pharmaceutical sciences (2023): 304-317
Staining of stratum corneum with fluorescent ε-poly-L-lysine and its application to evaluation of skin conditions.
Authors: Yoshida, Moemi and Numajiri, Sana and Notani, Nao and Sato, Nao and Nomoto, Koji and Arikawa, Hitomi and Urabe, Hiroya and Ichikawa, Hideyuki and Akimoto, Ryuji and Sato, Jun-Ichi and Yamashita, Yuji and Hirao, Tetsuji
Journal: Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] Internatio (2023): e13245
Agmatine production by Escherichia coli cells expressing SpeA on the extracellular surface.
Authors: Suzuki, Hideyuki and Thongbhubate, Kullathida and Muraoka, Madoka and Sasabu, Asuka
Journal: Enzyme and microbial technology (2023): 110139
Exclusion of Anchor-Matched Peptide Nucleic Acid from Liquid-Ordered Domains by Hybridization with Complementary Flavin-Labeled DNA.
Authors: Oka, Yoshimi
Journal: ACS omega (2023): 1109-1113

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18502920169
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029-68064558

AAT Bioquest ReadiCleave iFluor 488 SSL-NHS酯 货号7040
¥面议
¥面议
5mg可售

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